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#27705 Mouse/Rat Total Insulin (high sensitivity) Assay Kit - IBL

  • 27705 Mouse/Rat Total Insulin (high sensitivity) ELISA Kit
  • 27705 Mouse/Rat Total Insulin (high sensitivity) ELISA Kit
  • 27705 Mouse/Rat Total Insulin (high sensitivity) ELISA Kit
  • 27705 Mouse/Rat Total Insulin (high sensitivity) ELISA Kit
  • 27705 Mouse/Rat Total Insulin (high sensitivity) ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Mouse, Rat
Measuring Samples:
EDTA-plasma and Serum
Measurement Range:
1.25 ~ 80 pg/mL
Package Size1:
96 Well

Product Overview

Product Code 27705
Product Name Mouse/Rat Total Insulin (high sensitivity) Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Mouse, Rat
Measuring Samples EDTA-plasma and Serum
Measurement Range 1.25 ~ 80 pg/mL
Primary Reaction Overnight at 2 ~ 8°C
Secondary Reaction 60 minutes at 2 ~8°C
Sensitivity 0.50 pg/mL
Specificity Substance Cross Reactivity
GLP-1(7-36)amide ≦0.1%
GLP-1(7-37) ≦0.1%
GLP-1(9-36)amide ≦0.1%
GLP-1(1-37) ≦0.1%
Glucagon ≦0.1%
Oxyntomodulin ≦0.1%
Mouse GIP(1-42) ≦0.1%
Mouse GIP(3-42) ≦0.1%
Storage Condition 2~8°C
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Insulin is peptide hormone that is released from the beta cells of the islets of Langerhans of pancreas. 
It is synthesized as 86 amino-acid precursor “proinsulin” and it is cleaved by C-peptide and insulin that consists of 21 amino-acid A-chain and 30 amino-acid β chain that is linked by disulfide binding due to activation of prohormone conversion enzyme (PC2, PC3) in secretory granule.
Insulin deposits glucose and promotes synthesize protein in skeletal muscles. It also has physiological function such as suppress gluconeogenesis in liver and it has been known as an only hormone that can decrease blood glucose.    
Insulin is constantly released to maintaining the level of blood glucose while it is also released in large amount stimulated by incretin and glucose for suppressing rapidly increased blood glucose after meal.
However, lack of insulin secretion and decreasing insulin sensitivity are observed and eventually high blood glucose is observed in diabetic conditions.
Measuring insulin in fasting (F-IRI) is required for clinical assessment of diabetes such as function of insulin secretion and insulin resistance.
This ELISA kit can measure mouse and rat total insulin including proinsulin and low concentration of insulin in fasting using tiny amount of samples.    

The control set is available for sale on request. Learn More


Keywords:
GIP / GLP-1 / Insulin / Incretin

FAQ

  • Question
    Q.Does this kit detect Insulin I or Insulin II?
  • Answer
    A.This kit can be used for quantitatively measuring total insulin including Insulin I, Insulin II and Proinsulin.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
    (ELISA common FAQ * Specified FAQ for ELISA that is applicable for brain tissue extract.)
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.