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#27263 Human EL C-Terminal Assay Kit - IBL

  • 27263 Human EL C-Terminal ELISA Kit
  • 27263 Human EL C-Terminal ELISA Kit
  • 27263 Human EL C-Terminal ELISA Kit
  • 27263 Human EL C-Terminal ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, Serum
Measurement Range:
0.13 - 8 ng/mL
Package Size1:
96 Well

Product Overview

Product Code 27263
Product Name Human EL C-Terminal Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Serum
Measurement Range 0.13 - 8 ng/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 0.01 ng/mL
Specificity Substance Cross Reactivity
Human EL C-Terminal 100%
Human EL Full-Length 100%
Human LPL ≦ 0.1%
Human HTGL ≦ 0.1%
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Endothelial Lipase (EL) is a metabolic enzyme of HDL. It is phospholipase A1 molecule that is high substrate specificity against to phospholipid that exists in high density lipoprotein, HDL molecule and it promotes HDL metabolism through metabolizing HDL phospholipid.  It has been considered that EL involves with lipid metabolism and elevating risk of arteriosclerosis as strong expression of EL on newly formed vessel, macrophage in arteriosclerotic lesions is observed while EL expresses in vascular endothelial cells and vascular smooth myocytes of normal blood vessels.  It has been reported that both full length EL (active form) and cleaved EL (inactive forms, 40K fragment cleaved at N terminal and 28K fragment cleaved at C terminal cleaved by proprotein convertase) exist in blood. This ELISA kit is designed using two antibodies that detects C terminal fragments that can detect both full length EL and cleaved form EL that is cleaved at C-terminal in serum and EDTA-plasma regardless with or without administration of heparin injection that is conventionally required for the measurement.

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
  • Question
    Q.What is different between reagent blank and test sample blank?
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.