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#27710 Human Amyloidβ (1-43) (FL) Assay Kit - IBL

  • #27710 Human Amyloidβ (1-43) (FL) ELISA Kit
  • #27710 Human Amyloidβ (1-43) (FL) ELISA Kit
  • #27710 Human Amyloidβ (1-43) (FL) ELISA Kit
  • #27710 Human Amyloidβ (1-43) (FL) ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
CSF, Cell culture supernatant
Measurement Range:
2.34 - 150 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Code 27710
Product Name Human Amyloidβ (1-43) (FL) Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples CSF, Cell culture supernatant
Measurement Range 2.34 - 150 pg/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 60 minutes at 2 - 8 ℃
Specificity Compound Cross Reactivity
Human Aβ (1-43) 100.0%
Human Aβ (1-42) <1 %
Human Aβ (1-45) <0.1 %
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

The first case of Alzheimer's disease (AD) was defined and reported in 1907 by the German scientist, Dr. A. Alzheimer. His studies have shown that this is the main cause of dementia in the elderly. The plaques which appear in the brains of individuals who suffer AD are mostly constituted by the Amyloidβ protein (Aβ). Aβ is a peptide which consists of 40 or 42 (43) amino acids, and reports show that this is cleaved from β- and γ- secretase from the amyloid precursor protein. APP is a trans-membrane protein consisting of 695, 751, or 770 amino acids. Reports have shown many variants of Aβ exist and are clarified into the culture supernatant from the APP cDNA transfected mouse neuroblastoma cell. Many types of Aβ molecules are targeted in AD research. A process, Aβ40 is produced by cleaving from Aβ49 through Aβ46 and Aβ43, has been reported in tripeptide hypothesis, partly for that reason the interest in Aβ43 molecules has been growing. This kit can measure full-length (FL) molecules of Human Aβ (1-43).

Keywords:
Alzheimer's Disease / Amyloid β / Amyloid-β / Amyloid Beta / Aβ / Beta Amyloid

FAQ

  • Question
    Q.Can this kit be used for mouse or rat samples?
  • Answer
    A.No, it cannot.
    A monoclonal antibody (82E1) which is used as the labeled antibody in this kit, specifically reacts to N-terminal of Human Aβ.
    Since the N-terminal sequence of Aβ is different between human and rodents (mouse or rat), it does not cross reacts to mouse and/or rat Aβ.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.

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Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.