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#27729 Human Amyloidβ (1-x) Assay Kit - IBL

  • #27729 Human Amyloidβ (1-x) ELISA Kit
  • #27729 Human Amyloidβ (1-x) ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, Serum, CSF, Brain tissue extract, Cell culture supernatant
Measurement Range:
7.81 ~ 500 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Code 27729
Product Name Human Amyloidβ (1-x) Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Serum, CSF, Brain tissue extract, Cell culture supernatant
Measurement Range 7.81 ~ 500 pg/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 60 minutes at 2 - 8 ℃
Sensitivity 3.55 pg/mL
Specificity Compound Cross Reactivity
Human Aβ(1-40) 100.0%
Human Aβ(1-42) 100%
Human Aβ(1-16) ≦0.1%
Human Aβ(1-28) 100%
Human Aβ(-3-40) ≦0.1%
Human Aβ(-1-40) ≦0.1%
Human Aβ(2-40) ≦0.1%
Human Aβ(3-40) ≦0.1%
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

The first case of Alzheimer's disease was defined and reported in 1907 by the German scientist, Dr. A. Alzheimer. His studies have shown that this is the main cause of dementia in the elderly. The plaques which appear in the brains of Alzheimer's disease patients are mostly constituted by the Amyloid Beta (Amyloidβ, Aβ) protein. Amyloid Beta is a peptide which consists of 40 or 42 (43) amino acids, and reports show that this is cleaved from β- and γ- secretase from the amyloid precursor protein. APP is a trans-membrane protein consisting of 695, 751, or 770 amino acids. Reports have shown many variants of Amyloid Beta exist and are clarified into the culture supernatant from the APP cDNA transfected mouse neuroblastoma cell.Furthermore, in 1995, a dominant and differential deposition of distinct βamyloid peptide species, Amyloid Beta (N3pE), in senile plaques was found by Saido et al. This modified molecule, starting at the 3rd amino terminal residue, glutamate, was discovered to convert to pyroglutamate through intramolecular dehydration. The IBL Code No. 27713 can be measured Amyloid Beta (1-40) variants cleaved N terminal side by any cause. The IBL Code No. 27714, Human Amyloid Beta (1-40) (N) Assay Kit and IBL Code No. 27718 Human Amyloid Beta (1-40) (FL) Assay Kit are separately prepared when you want to measure Amyloid Beta (1-40) which held N terminal side completely. In addition, Human Amyloid Beta (1-40) (N) Assay Kit uses a polyclonal antibody as Labeled antibody. On the other hand, the Human Amyloid Beta (1-40) (FL) Assay Kit uses a monoclonal antibody. Moreover, in assay of Amyloid Beta (1-42) variants cleaved N terminal side, it is a Human Amyloid Beta (1-42) Assay Kit similarly. When measuring Amyloid Beta (1-42) variants cleaved by N terminal side, please use IBL Code No.27711, Human Amyloid Beta (1-42) Assay Kit. And, when measuring Amyloid Beta (1-42) which held N terminal side completely, please use IBL Code No.27712, Human Amyloid Beta(1-42) (N) Assay Kit. Furthermore, in measurement of Amyloid Beta (N3pE-42), please use IBL Code No.27716. Human Amyloid Beta (N3pE) assay Kit. Thus, it will be very useful for Alzheimer's research to assay Amyloid Beta (1-40), Amyloid Beta (1-42), and Amyloid Beta (N3pE-42), respectively. This kit can detect Amyloid Beta (1-x) variants which is cleaved at the C-terminal side by any cause, however, it can not detect variants which is smaller than Amyloid Beta (1-16). This kit uses a N-terminal specific monoclonal antibody as precoated antibody.

Keywords:
Alzheimer's Disease / Amyloid β / Amyloid-β / Amyloid Beta / Aβ / Beta Amyloid

FAQ

  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.

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Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.