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#27121 Human Fibulin-5/DANCE Assay Kit - IBL

  • 27121 Human Fibulin-5/DANCE ELISA Kit
  • 27121 Human Fibulin-5/DANCE ELISA Kit
  • 27121 Human Fibulin-5/DANCE ELISA Kit
  • 27121 Human Fibulin-5/DANCE ELISA Kit
  • 27121 Human Fibulin-5/DANCE ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, Cell culture supernatant
Measurement Range:
0.08 - 5.0 ng/mL
Package Size1:
96 Well

Product Overview

Product Code 27121
Product Name Human Fibulin-5/DANCE Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Cell culture supernatant
Measurement Range 0.08 - 5.0 ng/mL
Primary Reaction 60 minutes at 37 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 0.01 ng/mL
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

DANCE was identified as a ligand of integrins expressed mainly in arteries during embryogenesis and was named developmental arteries and neural crest EGF-like /DANCE. It is also called fibulin-5 and is a member of fibulin-family. Fibulin-5/DANCE is a secreted protein of 448 amino acids and 66kDa. It contains a RGD (arginine-glycine-asparticacid) motif in the N-terminal ragion and binds to integrins α5β1, αvβ3 and αvβ5. The expression of mRNA is augmented in smooth muscle cell, fibroblasts and endothelial cells of injured vascular tissues. Fibulin-5-deficient mice recapitulate human aging phenotypes, such as loose skin (cutis laxa), emphysematous lungs, and stiff ateries. It is known that destruction and degradation of elastic fibers directly cause many aging-related disorders like these. Disorganized elastic fibers were observed in the fibulin-5-deficient mice and indicating that fibulin-5/DANCE is an essential protein for elastogenesis. This product is an ELISA kit wich can measure human fibulin-5/DANCE.

The control set is available for sale on request. Learn More

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
  • Question
    Q.What is different between reagent blank and test sample blank?
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.