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#27782 soluble (Pro) renin Receptor Assay Kit - IBL

  • 27782 soluble (Pro) renin Receptor ELISA Kit
  • 27782 sPRR_human_urine_sample_caution for operations
  • 27782 sPRR_human_urine_sample_stability data (1)
  • 27782 sPRR_human_urine_sample_stability data (2)
  • 27782 soluble (Pro) renin Receptor ELISA Kit
  • 27782 sPRR_std_curve_human_serum
  • 27782 sPRR_std_curve_human_EDTA-plasma
  • 27782 sPRR_std_curve_human_urine
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human, Mouse, Rat
Measuring Samples：
Serum, EDTA-plasma, Urine (Human Only), Cell culture supernatant ※Please read the caution for operations (Pamphlet 2) if you measure sPRR in human urine samples.
Measurement Range：
125 - 8,000 pg/mL
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27782
Product Name soluble (Pro) renin Receptor Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human, Mouse, Rat
Measuring Samples Serum, EDTA-plasma, Urine (Human Only), Cell culture supernatant ※Please read the caution for operations (Pamphlet 2) if you measure sPRR in human urine samples.
Measurement Range 125 - 8,000 pg/mL
Primary Reaction Overnight at 2 - 8℃
Secondary Reaction 60 minutes at 2 - 8℃
Sensitivity 24 pg/mL
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well
Remarks1 Urine sample is only applicable for human sample. Mouse and rat urine samples are not suitable for measuring using this assay kit.

※Please read the caution for operations (Pamphlet 2) if you measure sPRR in human urine samples.

Product Description

Product Description

(Pro)renin receptor/(P)RR is a common receptor protein between renin and prorenin. When prorenin binds to (P)RR, it achieves a binding ability to angiotensinogen and gains a catalytic activity for conversion from angiotensinogen to ansiotensin I in comparable level with renin. Additionally, when the (P)RR is stimulated by binding to prorenin, its intracellular signaling progresses. Through this, (P)RR research is believed to have an important implication in building of new strategy for suppressing of overactivated tissue RAA (Renin-Angiotensin-Aldosterone) system. (P)RR is a 39 kDa, single transmembrane receptor protein and it is reported that 29 kDa soluble form is generated by cleavage of (P)RR with furin. Thus the quantitative assay of soluble (P)RR in blood or urine samples is expected to provide new knowledges to clarification of disease mechanisms and development of new tools for diseases. This ELISA kit can measure concentration of human soluble (pro)renin receptor in blood or urein samples.

References

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

FAQ

  • Question
    Q.What is the recommended dilution ratio for plasma samples?
  • Answer
    A.We recommend 40 fold dilution.
  • Question
    Q.What is recommended dilution ratio for human blood samples?
  • Answer
    A.It is 40 or more than 40 fold.
  • Question
    Q.Where the epitope (recognizing region) of antibodies used in this kit are?
  • Answer
    A.The recognizing region of the precoated polyclonal antibody (Anti- (Pro)renin receptor Rabbit IgG Affinity Purify) is located at the amino acid numbers of 148 to 165 in the sequence of (pro)renin receptor.
    The recognizing region of the HRP labeled monoclonal antibody (Anti- (P)RR (93A1B) Mouse IgG Fab’ Affinity Purify) is located at the amino acid numbers of 17 to 88 in the sequence of (pro)renin receptor.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
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