#27412 Human Total Angiotensinogen Assay Kit - IBL

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Intended Use：: Research reagents

Measuring Method：: ELISA

Sample Types：: Human

Measuring Samples：: Serum, EDTA-plasma, Urine, Cell culture supernatant　( ※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)

Measurement Range：: 0.31 ～ 20 ng/mL

Package Size1：: 96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Code	27412
Product Name	Human Total Angiotensinogen Assay Kit - IBL
Intended Use	Research reagents
Measuring Method	ELISA
Conjugate	HRP
Species	Human
Measuring Samples	Serum, EDTA-plasma, Urine, Cell culture supernatant　( ※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)
Measurement Range	0.31 ～ 20 ng/mL
Primary Reaction	60 minutes at 37 ℃
Secondary Reaction	30 minutes at 37 ℃
Sensitivity	0.03 ng/mL
Specificity	Substance Cross-reactivity Human Angiotensinogen 100.0% Angiotensin I < 0.1 % Angiotensin II < 0.1 % Angiotensin III < 0.1 % Angiotensin IV < 0.1 % Angiotensin (1-7) < 0.1 % Angiotensin (1-9) < 0.1 % Human albumin < 0.1 % Human IgG < 0.1 % Human Angiopoietin-like 3 < 0.1 %
Storage Condition	2 - 8 ℃
Poisonous and Deleterious Substances	Not Applicable
Cartagena	Not Applicable
Measuring Service	Not Available
Package Size 1	96 Well
Remarks1	An additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the details on the datasheet. As the composition of EIA buffer may be different on each product, please use the following product specific EIA buffer for additional use. Product Code: 27412D100 Product Name: Human Angiotensinogen EIA buffer 100 Size: 100 mL

Product Description

Angiotensinogen is the precursor of angiotensin and is cleaved into angiotensin I and II in the renin-angiotensin system, and it has long been reported to play an important role in controlling blood pressure. In recent years interest related to the role of the renin-angiotensin system in arterial pressure control and the pathophysiology of hypertension has been shifting to its local role in various tissues. Among the studies urinary excretion of angiotensinogen in a rat model of angiotensin II (AII)-dependent hypertension has been reported to be a marker of the activity of the local intrarenal renin-angiotensin system. Intrarenal AII increases to an extent in AII-dependent hypertension that cannot be explained by the plasma AII equilibration alone, and two mechanisms, an increase in intracellular uptake of AII and an increase in intrarenal expression of angiotensinogen, have been proposed to explain it.

The kit can quantitatively measure Human Total Angiotensinogen.

The control set is available for sale on request. Learn More

Keywords:
RAAS / ACE / Angiotensinogen / Angiotensin II / AGT

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

Q.What are the epitopes of the antibodies used in this kit?
A.The epitope of the pre-coated antibody (Anti-Human AGT (72) Rabbit IgG Affinity Purify) is IQAKTSPVDEKALQDQLVLV. We do not have the epitope for HRP conjugated Anti- Human AGT (601) Mouse IgG, but the detection part is around C-terminal.
Q.What is the best time for collecting urine?
A.We recommend collecting urine on an as needed basis.
Q.How should urine samples be stored after they are collected?
A.They should be stored at -80°C immediately after they are collected.
Q.How many minutes should the urine samples be centrifuged, and at what rpm rate?
A.This process is not required.
Q.Do you have any data of normal value of total Angiotentinogen in human blood and standard range?
A.Please refer to the following article. https://www.ncbi.nlm.nih.gov/pubmed/?term=17553939
Q.Can dog sample be used for this kit?
A.The epitope of human and dog are quite different. Therefore, the kit cannot be used for dog sample.
Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
ELISA common FAQ
A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
Q.What is the composition of concentrated wash buffer?
ELISA common FAQ
A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
Q.What is the feature of the plate?
ELISA common FAQ
A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
Q.Can I re-use standard after reconstitution?
ELISA common FAQ
A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
Please check the details on each product datasheet.
Q.What is different between reagent blank and test sample blank?
ELISA common FAQ
A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
Q.How many samples can be measured by this kit?
ELISA common FAQ
A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
Q.What is LOD (Limit of Detection)?
ELISA common FAQ
A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
Q.What is LOQ (Limit of Quantification)?
ELISA common FAQ
A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
Q.What is the definition of Over Night (O/N) reaction?
ELISA common FAQ
A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
Q.What is the specification of quality control for ELISA product release?
ELISA common FAQ
A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
ELISA common FAQ
A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
Q.How to wash an ELISA plate?
ELISA common FAQ
A.Washing it by an auto-washer is highly recommended.
If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
Q.The wells turned black during the test with the kit.
ELISA common FAQ
A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
Q.How do you define the acceptance range of quality control of control sets?
Control Set Common FAQ
A.In principal, it is defined as ±15%-20% based the standard value described on each datasheet.
Q.What is the shelf life of control set?
Control Set Common FAQ
A.The shelf life is 12 months upon the goods is dispatched from our lab if the goods is unopned.
Q.What is the storage condition of control sets?
Control Set Common FAQ
A.It should be stored under chilled condition (2～10℃).
Q.Can the control set be used after reconstitution?
Control Set Common FAQ
A.No it cannot be re-used after the reconstitution.
Q.What is the package form of control sets?
Control Set Common FAQ
A.It is lyophilized form.