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#27105 Mouse Intact Angiotensinogen Assay Kit - IBL

  • 27105 Mouse Intact Angiotensinogen ELISA Kit
  • 27105 Mouse Intact Angiotensinogen ELISA Kit
  • 27105 Mouse Intact Angiotensinogen ELISA Kit
  • 27105 Mouse Intact Angiotensinogen ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Mouse
Measuring Samples:
Serum, EDTA-plasma (※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)
Measurement Range:
0.16 - 10 ng/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27105
Product Name Mouse Intact Angiotensinogen Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Mouse
Measuring Samples Serum, EDTA-plasma (※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)
Measurement Range 0.16 - 10 ng/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 60 minutes at 2 - 8 ℃
Sensitivity 0.01 ng/mL
Specificity Specifically detect intact Angiotensinogen in Mouse serum and EDTA-plasma.
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well
Remarks1 An additional EIA buffer may be required depending on your samples.
The EIA buffer is available with charge. Please refer to the details on the datasheet.
As the composition of EIA buffer may be different on each product, please use the following product specific EIA buffer for additional use.

Product Code: 27105D100
Product Name: Mouse Intact Angiotensinogen EIA buffer 100
Size: 100 mL
Remarks2 OPERATING PRECATION
Wash the plate immediately after each reaction using by a plate washer with setting wait time zero second.
The O.D. value tends to be lower if washing time is getting longer.

Product Description

Product Description

Angiotensinogen is a precursor protein of Angiotensin and it is cleaved by renin enzyme secreted from juxtaglomerular cells (JG Cells) located at afferent arterioles of kidneys, and Angiotensin I is produced. After that it is cleaved by Angiotensin-Converting Enzyme (ACE) and Angiotensin II is produced.

In the Renin-Angiotensin-Aldosterone System (RAAS), Angiotensin II has an important role of arterial contraction of the entire body, and stimulates adrenal cortex to secret Aldosterone. Aldosterone deposits Na in the body and because of the feature, the volume of blood circulation, cardiac output and peripheral vascular resistance (PVR) are increased. Renin is inhibited after blood pressure is increased.

In the past, plasma renin activity (PRA), active renin concentration (ARC) and its combination were used for assessment of renin activity in blood marker in RAAS, however, the impact of posture such as erect position or seated position, and also variability in a day were not properly considered in the assessment. According to study lead by Kobori et al., it has been suggested that the ratio of des AI Angiotensinogen / Intact Angiotensinogen can be calculated through the measurement of both Intact Angiotensinogen and total angiotensinogen in blood and it can be much stabile renin activity biomarker.

Measurement of Angiotensinogen is useful in research such of primary aldosteronism, novel RAAS inhibitor and cardiovascular and renovascular disease.

More recently, the association between COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and RAAS including ACE inhibitors and Angiotensin receptor inhibitors (ARBs) has been actively researched around the world.

This kit can quantitatively measure Intact Angiotensinogen (Full Length Angiotensinogen) in mouse serum and plasma samples.

Keywords:
RAAS / ACE / Angiotensinogen / Angiotensin II