Product Search

#27103 Mouse Total Angiotensinogen Assay Kit - IBL

  • 27103 Mouse Total Angiotensinogen ELISA Kit
  • 27103 Mouse Total Angiotensinogen ELISA Kit
  • 27103 Mouse Total Angiotensinogen ELISA Kit
  • 27103 Mouse Total Angiotensinogen ELISA Kit
  • 27103 Mouse Total Angiotensinogen ELISA Kit
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Mouse
Measuring Samples：
Serum, EDTA-plasma, Cell culture supernatant. (※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)
Measurement Range：
0.16 - 10 ng/mL
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27103
Product Name Mouse Total Angiotensinogen Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Mouse
Measuring Samples Serum, EDTA-plasma, Cell culture supernatant. (※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)
Measurement Range 0.16 - 10 ng/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 60 minutes at 2 - 8 ℃
Sensitivity 0.03 ng/mL
Specificity Specifically detect Total Angiotensinogen in Mouse serum, EDTA-plasma and cell culture supernatant.
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well
Remarks1 An additional EIA buffer may be required depending on your samples.
The EIA buffer is available with charge. Please refer to the details on the datasheet.
As the composition of EIA buffer may be different on each product, please use the following product specific EIA buffer for additional use.

Product Code: 27103D100
Product Name: Mouse Angiotensinogen EIA buffer 100
Size: 100 mL
Remarks2 OPERATING PRECATION
Wash the plate immediately after each reaction using by a plate washer with setting wait time zero second.
The O.D. value tends to be lower if washing time is getting longer.

Product Description

Product Description

Angiotensinogen is a precursor protein of Angiotensin and it is cleaved by renin enzyme secreted from juxtaglomerular cells (JG Cells) located at afferent arterioles of kidneys, and Angiotensin I is produced. After that it is cleaved by Angiotensin-Converting Enzyme (ACE) and Angiotensin II is produced.

In the Renin-Angiotensin-Aldosterone System (RAAS), Angiotensin II has an important role of arterial contraction of the entire body, and stimulates adrenal cortex to secret Aldosterone. Aldosterone deposits Na in the body and because of the feature, the volume of blood circulation, cardiac output and peripheral vascular resistance (PVR) are increased. Renin is inhibited after blood pressure is increased.

In the past, plasma renin activity (PRA), active renin concentration (ARC) and its combination were used for assessment of renin activity in blood marker in RAAS, however, the impact of posture such as erect position or seated position, and also variability in a day were not properly considered in the assessment. According to study lead by Kobori et al., it has been suggested that the ratio of des AI Angiotensinogen / Intact Angiotensinogen can be calculated through the measurement of both Intact Angiotensinogen and total angiotensinogen in blood and it can be much stabile renin activity biomarker.

Measurement of Angiotensinogen is useful in research such of primary aldosteronism, novel RAAS inhibitor and cardiovascular and renovascular disease.

More recently, the association between COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and RAAS including ACE inhibitors and Angiotensin receptor inhibitors (ARBs) has been actively researched around the world.

This kit can quantitatively measure Total Angiotensinogen (both Intact Angiotensinogen and Des AI Angiotensinogen cleaved by renin) in mouse serum, plasma and Cell culture supernatant samples.

Keywords:
RAAS / ACE / Angiotensinogen / Angiotensin II  / AGT

FAQ

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
Contact Us >