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#27742 Human Intact Angiotensinogen Assay Kit - IBL
- Intended Use:
- Research reagents
- Measuring Method:
- ELISA
- Sample Types:
- Human
- Measuring Samples:
- Serum, EDTA-plasma, Urine (Recommend to use spot urine samples) ( ※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)
- Measurement Range:
- 0.28 - 18 ng/mL
- Package Size1:
- 96 Well
※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
enclosed in the product purchased before use.
Product Overview
Product Overview
Product Code | 27742 |
---|---|
Product Name | Human Intact Angiotensinogen Assay Kit - IBL |
Maker Name | Immuno-Biological Laboratories Co., Ltd. |
Intended Use | Research reagents |
Measuring Method | ELISA |
Conjugate | HRP |
Species | Human |
Measuring Samples | Serum, EDTA-plasma, Urine (Recommend to use spot urine samples) ( ※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.) |
Measurement Range | 0.28 - 18 ng/mL |
Primary Reaction | 60 minutes at 37 ℃ |
Secondary Reaction | 30 minutes at 37 ℃ |
Sensitivity | 0.093 ng/mL |
Specificity | Specifically detect Human Intact Angiotensinogen. Angiotensinogen taken apart doesn't detect it in renin. |
Storage Condition | 2 - 8 ℃ |
Poisonous and Deleterious Substances | Not Applicable |
Cartagena | Not Applicable |
Measuring Service | Available |
Package Size 1 | 96 Well |
Remarks1 | ※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details. The following product is available with charge if an additional buffer is required. Please note, this buffer can be only used for #27742. Product Code: 27742D100 Product Name: Human Intact Angiotensinogen EIA buffer 100 Size: 100 mL |
Product Description
Product Description
Angiotensinogen is the precursor of angiotensin and is cleaved into angiotensin I and II in the renin-angiotensin system, and it has long been reported to play an important role in controlling blood pressure. In recent years interest related to the role of the renin-angiotensin system in arterial pressure control and the pathophysiology of hypertension has been shifting to its local role in various tissues. Among the studies urinary excretion of angiotensinogen in a rat model of angiotensin II (AII)-dependent hypertension has been reported to be a marker of the activity of the local intrarenal renin-angiotensin system. Intrarenal AII increases to an extent in AII-dependent hypertension that cannot be explained by the plasma AII equilibration alone, and two mechanisms, an increase in intracellular uptake of AII and an increase in intrarenal expression of angiotensinogen, have been proposed to explain it.
This kit can quantitatively measure Human Intact (Full-Length) Angiotensinogen in Serum, EDTA-plasma and Urine.
These samples should be diluted with appropriate dilution ratio prior conducting the assay. The dilution ratio should be determined by each laboratory.
In that case the EIA buffer” in the kit is not enough for dilution, it can be separately purchased with charge (100 mL, Code No. 27742D100).
The control set is available for sale on request. Learn More
Keywords:
RAAS / ACE / Angiotensinogen / Angiotensin II / AGT
This kit can quantitatively measure Human Intact (Full-Length) Angiotensinogen in Serum, EDTA-plasma and Urine.
These samples should be diluted with appropriate dilution ratio prior conducting the assay. The dilution ratio should be determined by each laboratory.
In that case the EIA buffer” in the kit is not enough for dilution, it can be separately purchased with charge (100 mL, Code No. 27742D100).
The control set is available for sale on request. Learn More
Keywords:
RAAS / ACE / Angiotensinogen / Angiotensin II / AGT
References
References
- Evidence for Impaired Renin Activity in Postural Orthostatic Tachycardia Syndrome. Spahic JM et al. J Clin Med. 2023 Jul 13;12(14):4660.PMID: 37510775
- Renal Angiotensinogen is Predominantly Liver-Derived in Nonhuman Primates. Kukida M et al. Arterioscler Thromb Vasc Biol. 2021 Nov;41(11):2851-2853.PMID: 34496634
Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov
FAQ
FAQ
-
Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
ELISA common FAQ -
A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
-
Q.What is the composition of concentrated wash buffer?
ELISA common FAQ -
A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
-
Q.What is the feature of the plate?
ELISA common FAQ -
A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
-
Q.Can I re-use standard after reconstitution?
ELISA common FAQ -
A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
Please check the details on each product datasheet. -
Q.What is different between reagent blank and test sample blank?
ELISA common FAQ -
A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
-
Q.How many samples can be measured by this kit?
ELISA common FAQ -
A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
-
Q.What is LOD (Limit of Detection)?
ELISA common FAQ -
A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
-
Q.What is LOQ (Limit of Quantification)?
ELISA common FAQ -
A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
-
Q.What is the definition of Over Night (O/N) reaction?
ELISA common FAQ -
A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
-
Q.What is the specification of quality control for ELISA product release?
ELISA common FAQ -
A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
-
Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
ELISA common FAQ -
A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
-
Q.How to wash an ELISA plate?
ELISA common FAQ -
A.Washing it by an auto-washer is highly recommended.
If it is not available, please refer to the demo video (only 2 mins) using a washing bottle. -
Q.The wells turned black during the test with the kit.
ELISA common FAQ -
A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL. -
Q.How do you define the acceptance range of quality control of control sets?
Control Set Common FAQ -
A.In principal, it is defined as ±15%-20% based the standard value described on each datasheet.
-
Q.What is the shelf life of control set?
Control Set Common FAQ -
A.The shelf life is 12 months upon the goods is dispatched from our lab if the goods is unopned.
-
Q.What is the storage condition of control sets?
Control Set Common FAQ -
A.It should be stored under chilled condition (2~10℃).
-
Q.Can the control set be used after reconstitution?
Control Set Common FAQ -
A.No it cannot be re-used after the reconstitution.
-
Q.What is the package form of control sets?
Control Set Common FAQ -
A.It is lyophilized form.