FAQ Antibody - IBL
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Antibody - IBL （）
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Q.Is endogenic peroxidase treatment required for fluorescence stain? -
A.No, it is not required. -
Q.Regarding formic acid treatment, is inactivation treatment by citric acid autoclave required?
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A.No, inactivation treatment by citric acid autoclave is not required. However, we recommend formic acid treatment (after deparaffinization, leave it in formic acid for 5 to 30 minutes and wash it by running water.) if you use brain tissue.
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Q.Can it be used for IHC for mouse and rat samples? What kind of positive control was used for?
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A.Yes, it can. We use human lung adenocarcinoma and human stomach cancer tissues for positive control.
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Q.It seems like this antibody can detect apotosis cells, but does it also positively react to other stage of cells such as mitotic period phase cells or ischemia-induced dead cells?
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A.We consider that it does only detect apotosis cells due to its specificity.
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Q.What is the sequence of the antigen or where is the region?
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A.The sequence is IPVKQADSGSSEEKQ. The region is N-terminal of full length of Osteopontin.
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Q.Does this antibody detect full length Osteopontin or Osteopontin cleaved by Thrombin? If the antibody detects Osteopontin cleaved by Thrombin, which part of cleaved Osteopontin is detected by this antibody?
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A.As the region of the antigen is N-terminal of full length Osteopontin, the antibody detects both full length Osteopontion and N-terminal Osteopontin cleaved by Thrombin. This antibody does not detect to C-terminal Osteopontin cleaved by Thrombin.
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Q.What is the epitope?
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A.It is IPVKQADSGSSEEKQ.
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Q.What can be used for positive control?
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A.Cancer cellline such as NB-1 can be used as the positive control.
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Q.What can be used for positive control?
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A.Cancer cellline such as NB-1 can be used as the positive control.
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Q.What is the condition of dilution when it is used for IHC?
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A.Recommended concentartion for use is 5μg/mL. Please dilute reconstituted 1mL (100μg/mL) 20 folds for use.
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Q.Can this antibody be used on human or mice samples?
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A.It has not been tested.
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Q.What species of monkey is used for absorbing this product?
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A.The crab-eating macaque (cynomolgus monkey).
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Q.How do you biotinylation the antibody?
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A.NHS-LC-Biotin is used for the biotinylation.
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Q.Can this antibody be used on mice or rat samples ?
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A.No it cannot.
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Q.Does the antibody cross react to mice?
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A.No, it doesn't.
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Q.Can this antibody be used to stain the paraffin section?
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A.No, it cannot be used to stain this section.
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Q.Can this antibody use for rat samples?
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A.As only one amino-acid is different between mouse and rat, so it would be able to use for rat but we cannot be certain for it. Unfortunately, we do not have any experiences nor data for rat sample wit hte antibody.
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Q.Does it cross react to human Claudin-6?
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A.Yes, it does due to the sequence is same between mouse and human. SRGPSEYPTKNYV
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Q.Can this antibody stain frozen sections?
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A.No it cannot.
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Q.Which APP types can be detected by this antibody?
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A.This antibody can detect any isoform of APP.
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Q.Can this antibody be used on mice samples ?
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A.No, it cannot.
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Q.Which region can this antibody recognize?
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A.The extracellular domain.
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Q.Can this antibody stain frozen sections?
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A.Yes it can.
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Q.Can this antibody be used on mice samples?
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A.No it cannot.
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Q.Can this antibody be used on mice samples ?
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A.No, it cannot.
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Q.Can this antibody be used for FACS?
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A.No, it cannot.
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Q.Can this antibody be used on mice samples ?
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A.Yes, it can.
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Q.Can this antibody be used on mice or rat samples ?
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A.No, they cannot.
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Q.Which immunizing antigen is used?
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A.A fragment of the N terminal of Nephrin (amino-acid 22-240) is used as the antigen.
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Q.Can this antibody be used for staining?
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A.No it cannot.
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Q.Can this antibody be used on mice samples ?
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A.No, it cannot.
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Q.Can this antibody be used for staining?
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A.No it cannot.
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Q.What is the molecule size?
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A.Approximately 52kDa.
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Q.Can this antibody detect full length of APP or sAPPα?
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A.This antibody cannot react with the full length of APP or sAPPα when performing evaluations with WB. However, because we have only conducted evaluations with WB, we cannot be 100% certain about the cross reactivity with sAPPα and full-length APP. That is why we made the following statement on our datasheet: "This antibody can detect the sAPP-Wild type, which is cleaved by β-secretase. It exhibits very little cross-reaction with sAPPα and full-length APP."
