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#27742 Human Intact Angiotensinogen Assay Kit - IBL

  • 27742 Human Intact Angiotensinogen ELISA Kit
  • 27742 Human Intact Angiotensinogen ELISA Kit
  • 27742 Human Intact Angiotensinogen ELISA Kit
  • 27742 Human Intact Angiotensinogen ELISA Kit
  • 27742 Human Intact Angiotensinogen ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
Serum, EDTA-plasma, Urine (Recommend to use spot urine samples) ( ※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)
Measurement Range:
0.28 - 18 ng/mL
Package Size1:
96 Well

Product Overview

Product Code 27742
Product Name Human Intact Angiotensinogen Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Serum, EDTA-plasma, Urine (Recommend to use spot urine samples) ( ※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)
Measurement Range 0.28 - 18 ng/mL
Primary Reaction 60 minutes at 37 ℃
Secondary Reaction 30 minutes at 37 ℃
Sensitivity 0.093 ng/mL
Specificity Specifically detect Human Intact Angiotensinogen.
Angiotensinogen taken apart doesn't detect it in renin.
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well
Remarks1 ※ Additional EIA buffer may be required depending on your samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.

The following product is available with charge if an additional buffer is required. Please note, this buffer can be only used for #27742.

Product Code: 27742D100
Product Name: Human Intact Angiotensinogen EIA buffer 100
Size: 100 mL

Product Description

Angiotensinogen is the precursor of angiotensin and is cleaved into angiotensin I and II in the renin-angiotensin system, and it has long been reported to play an important role in controlling blood pressure. In recent years interest related to the role of the renin-angiotensin system in arterial pressure control and the pathophysiology of hypertension has been shifting to its local role in various tissues. Among the studies urinary excretion of angiotensinogen in a rat model of angiotensin II (AII)-dependent hypertension has been reported to be a marker of the activity of the local intrarenal renin-angiotensin system. Intrarenal AII increases to an extent in AII-dependent hypertension that cannot be explained by the plasma AII equilibration alone, and two mechanisms, an increase in intracellular uptake of AII and an increase in intrarenal expression of angiotensinogen, have been proposed to explain it.

This kit can quantitatively measure Human Intact (Full-Length) Angiotensinogen in Serum, EDTA-plasma and Urine.

These samples should be diluted with appropriate dilution ratio prior conducting the assay. The dilution ratio should be determined by each laboratory.

In that case the EIA buffer” in the kit is not enough for dilution, it can be separately purchased with charge (100 mL, Code No. 27742D100).

The control set is available for sale on request. Learn More

 

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
    (ELISA common FAQ * Specified FAQ for ELISA that is applicable for brain tissue extract.)
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.