Product related FAQ

A.It does react to both intact and cleaved isoform (E41) of α2, 6-Sialyltransferase.

A.The antibody both phosphorylated and non-phosphorylated Tob1 detects.

A.As the amino acid sequence of Tob1 is 345 a.a, it is approx. 38kDa based on the calculation.
The molecular size of phosphorylated Tob1 is bigger than non-phosphorylated and the size is suggested as around 45kDa - 50kDa.

A.It is SVVYGLR.

A.It is KSKKFRRPDIQYPDATDE.

A.It is TVELAELGPLLEEKGKRV.

A.No, it cannot.

A.It detects VEGF165 and VEGF121.

A.It is GVVIA.

A.Yes, it can. This antibody was used in the following publication. Please refer to it.

Requirements for the functional expression of OX40 ligand on human activated CD4+ and CD8+ T cells.
Kondo K et al. Hum Immunol. 2007 Jul;68(7):563-71. Epub 2007 Apr 13.

A.Yes, it can.

A.As the catalog product contains BSA and NaN3, the antibody cannot be used as neutralizing antibody.
However, if we specifically produce the antibody as customized product without BSA and NaN3 and sterilization for filling process in frozen, it can be used as neutralizing antibody.
Please feel free to contact us if you are interested in the customized antibody.

A.No, it cannot be recommended for the use. A biotinized antibody is recommended to use for the purpose.

A.NHS-LC-Biotin is used for the biotinylation.

A.The subclass is Rat IgG1. The light chain is lambda.

A.NHS-LC-Biotin is used for the biotinylation.

A.No, it doesn't.

A.As the antibody specifically reacts N-terminal of Amyloidβ, it does not react to APP, Aβ(2-40) and Aβ(3-40).

A.NHS-LC-Biotin is used for the biotinylation.

A.As the antibody specifically reacts N-terminal of Amyloidβ, it does not react to APP, Aβ(2-40) and Aβ(3-40).

A.It is 100μg/mL.

A.KM55 is 100ug/1mL.
Usually, 0.1-0.2mL/preparate is used. This means 5-10 prepalates can be stained.

A.Please refer to the method written in this article.

A.Total IgA is approx 1-4mg/mL in blood.

A.As it is approx 1,000 ng/mL in blood, more than 200 fold dilution is recommended for measurement.

A.It recognizes PST(GalNAc)PP.
Please refer to this article.

A.The targeted antigen might be masked by the fixation process in immunohistochemical staining.
For avoiding such masking effects, immunogen activation (enzymatic treatment or heat treatment) is required for exposing the targeted antigen.
Subtilisin A treatment is recommended for IHC using the antibody (KM55).

A.The antigen, GST-TobN that is used in the article is fusion protein of N-terminal 168 amino acids of Tob protein and GST.
The sequence of this region is same between human and mouse.
Therefore, theoretically it would be possible to detect using by the antibody #18911 with mice sample but as we have not tested, we cannot be certain about the point.

A.The crab-eating macaque (cynomolgus monkey).

A.Cross reactivity with mouse samples is not so strong when it's compared with human samples. But we have confirmed this antibody detects mouse sAPPα.

A.Recombinant 27A and 27B (lysates of transfectant cell) are used as the positive control.

A.The position is between 65 and 120 of amino acid sequences of human Galectin-3.

A.The position is between 65 and 120 of amino acid sequences of human Galectin-3.

A.The antibody recognizes the region contains arginine located at position 112 of amino acid sequences of human ApoE4.

A.No, it is not required.

A.No, inactivation treatment by citric acid autoclave is not required. However, we recommend formic acid treatment (after deparaffinization, leave it in formic acid for 5 to 30 minutes and wash it by running water.) if you use brain tissue.

A.Yes, it can. We use human lung adenocarcinoma and human stomach cancer tissues for positive control.

A.We consider that it does only detect apotosis cells due to its specificity.

A.The sequence is IPVKQADSGSSEEKQ. The region is N-terminal of full length of Osteopontin.

A.As the region of the antigen is N-terminal of full length Osteopontin, the antibody detects both full length Osteopontion and N-terminal Osteopontin cleaved by Thrombin. This antibody does not detect to C-terminal Osteopontin cleaved by Thrombin.

A.It is IPVKQADSGSSEEKQ.

A.Cancer cellline such as NB-1 can be used as the positive control.

A.Cancer cellline such as NB-1 can be used as the positive control.

A.Recommended concentartion for use is 5μg/mL. Please dilute reconstituted 1mL (100μg/mL) 20 folds for use.

A.It has not been tested.

A.The crab-eating macaque (cynomolgus monkey).

A.NHS-LC-Biotin is used for the biotinylation.

A.No it cannot.

A.No, it doesn't.

A.No, it cannot be used to stain this section.

A.As only one amino-acid is different between mouse and rat, so it would be able to use for rat but we cannot be certain for it. Unfortunately, we do not have any experiences nor data for rat sample wit hte antibody.

A.Yes, it does due to the sequence is same between mouse and human. SRGPSEYPTKNYV

A.No it cannot.

A.This antibody can detect any isoform of APP.

A.No, it cannot.

A.The extracellular domain.

A.Yes it can.

A.No it cannot.

A.No, it cannot.

A.No, it cannot.

A.Yes, it can.

A.No, they cannot.

A.A fragment of the N terminal of Nephrin (amino-acid 22-240) is used as the antigen.

A.No it cannot.

A.No, it cannot.

A.No it cannot.

A.Approximately 52kDa.

A.This antibody cannot react with the full length of APP or sAPPα when performing evaluations with WB. However, because we have only conducted evaluations with WB, we cannot be 100% certain about the cross reactivity with sAPPα and full-length APP. That is why we made the following statement on our datasheet: "This antibody can detect the sAPP-Wild type, which is cleaved by β-secretase. It exhibits very little cross-reaction with sAPPα and full-length APP."

A.No, it cannot.

A.No, it cannot.

A.No, it cannot.

A.The antibody reacts to 165, 189, and 206.

A.yes, it can.

A.No, it cannot.

A.ET-AR appears around 48kDa, and ET-BR appears around 50kDa.

A.No it cannot.

A.The thrombin-cleaved C-half can be detected, but the N-half cannot.

A.No, it doesn’t.

A.No, it doesn't.